PT. ERA MAJU SEJAHTERA

R2-M Flow-through System

Specifications

  • Physical dimensions : 350mm x 360mm x 148mm
  • Actual Weight : 3.5kg
  • Temperature Accuracy : +/- 0.5C
  • Power Requirement : 150W
  • Pump rate : 50ml per-minute
  • Reaction chamber size : 70mmx106mm

Specifications

  • Physical dimensions : 350mm x 360mm x 148mm
  • Actual Weight : 3.5kg
  • Temperature Accuracy : +/- 0.5C
  • Power Requirement : 150W
  • Pump rate : 50ml per-minute
  • Reaction chamber size : 70mmx106mm

 


 

Flow-through Hybridization Technology provides the platform for rapid analyses of analyte in the Solution-Porous-Matrix System. This is exemplified by the DNA hybridization: It is characterized by active approach to facilitate the annealing reaction between the sample molecules (targets) and the capturing molecules (probes), which occur on three-dimensional porous matrixes rather than planar surfaces.

The annealing of two complementary DNA single strands is very fast kinetic process when the two strands collide. However in the two-phase conventional hybridization system, the target molecules must move forwars the probe fixes in the solid matrix during hybridization.

Hence this diffusion through solid matrix – a slow process – is the rate-limiting step and therefore the incubation step usually takes hours or even overnight in order to achieve optimal result.

 


 

The “Flow-through” process overcomes this diffusion limitation during the annealing process and all subsequent steps as required for signal generation. By directing the targets toward the probes to form duplexes, the hybridization is changed from the passive and random diffusion to active channeling process allowing the recombination reactions to complete in seconds.

The washing of non-specific and unbounded targets will be removed similarly. Theoretically, the “Flow-through” method will produce the optimal signal and signal-to-noise ratios rapidly for affinity capturing chemical processes: i.e. DNA-DNA; DNA-protein; Antibody-antigens; Ligand-receptor and other molecular interactions.

Added Values of “Flow-through” Process

  • Ease of operation
  • Shorten hybridization and other processes duration
  • Simple solid support: Membrane i.e. Nylon membrane
  • Less reagent consumption
  • Open architectural paltform
  • Multiple analytes detection
  • Adoptable to varies signal detection methodologies
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